Thursday, 4th February 26th Annual Lorne Proteomics Symposium 2021

• Multi-omics to explore bacterial virulence and vaccine design - Stuart J. Cordwell

• Methylation, phosphorylation and their crosstalk on translation-associated proteins - Joshua J Hamey

•   Cathelicidin-3 enriched extracellular vesicles as preclinical biomarker for Tasmanian Devil Facial Tumour Disease - Camila Espejo
• Integration of immunopeptidomics and functional immunology for the identification of drug-induced T cell epitopes - Johanna EE Tuomisto
• Identification of novel proteins encoded by the human genome - Hitesh Kore
• Multi-omics of B-cell precursor acute lymphoblastic leukemia cells with MLL rearrangement (MLL‑r) revealed a deep modification of their glycosylation machinery - Tiago Oliveira

• PREDICTING AND MONITORING CHEMORESPONSE USING PATIENT DERIVED SAMPLES OF OVARIAN CANCER - Manuela Klingler-Hoffmann
• Clinical Proteomics - improving outcomes for children - Vera Ignjatovic
• Moving diagnostic quantitative proteomics for rare disease from the bench to the clinic - David A Stroud
• A multi-omic approach to study interactions between the gut virome, microbiome and metaproteome during development of islet autoimmunity - Emma E Hamilton-Williams
• Multi-omics approach to identify cancer neo-antigens for immunotherapy - Harsha Gowda

Sponsor Lunchtime Workshop

Multidimensional data extraction from scanning quadrupole SWATH data ​

Presenter: Stephen Tate, Software and Applications Research Manager, SCIEX ​

Abstract: ​

Novel Data Independent Acquisition methods are provide opportunities for multidimension data deconvolution using the both the fragment ions profile from the isolation quadrupole and the LC dimension. These enhancements provide increased confidence in data assignment over standard Data Independent Analysis methods.  Scanning methods operate at fast cycle times with data collection rates equivalent to the pulse frequency of TOF instruments. Using these fast data rates it is possible to assign fragments to all parent ions providing an accurate picture of the sample. ​

As the resolution of the quadrupole scan is greater than the normal quadrupole isolation width we will show mathematical deconvolution of co-isolating precursors in DDA, and spectral deconvolution of pesticide mixtures which are equivalent to standard library spectra. ​

• SWATH glycoproteomics of sparkling wine - Cassandra Pegg
• Protein extraction protocols for optimal proteome measurement and arginine kinase quantitation from cricket Acheta domesticus - Michelle Colgrave
• Phosphoproteome rearrangement of rice leaves in prolonged water stress and varying nitrogen supplementation - Sara Hamzelou
• Targeted quantitative analysis of conglutin seed storage proteins in lupin seeds - Arineh Tahmasian
• Quantitative proteomics to investigate rabbit haemorrhagic disease virus infection - Elena Smertina

• Proteomics of developing cotton pollen reveals temporal patterns under heat stress - Farhad Masoomi-Aladizgeh
• Comprehensive proteomic profiling of blood extracellular vesicles via SWATH mass spectrometry: a new avenue for glioma tumour monitoring - Susannah Hallal
• Proteomic analysis of adipose depots after intermittent fasting reveals visceral fat preservation mechanisms - Dylan J Harney
• DIA based plasma proteomics for detection of diabetes related pathologies: readiness to support the FIELD clinical trial - Matthew B O'Rourke
• GlypNirO: An automated workflow for quantitative N- and O-linked glycoproteomic data analysis - Toan K. Phung
• The post translational modification landscape of commercial beers using quantitative proteomics - Edward D Kerr
• Illuminating the dark lipidome of cancer using isomer-resolved lipidomics - Reuben SE Young
• A novel approach for biomarker discovery: spatial mapping of N-glycans on human knee osteoarthritis cartilage-bone tissue using matrix-assisted laser desorption/ionisation mass spectrometry imaging - Yea Rin Olivia Lee

• In vivo phosphoproteomics of age-associated insulin-signalling in bone tissue - Benjamin Parker
• Temporal quantitative proteomics and phosphoproteomics reveals ubiquitin proteasome system is essential to mount adaptive response to hypoxia in melanoma cells - Keshava Datta
• Controlling the controllers: the post-translational regulation of histone methylation enzymes in yeast - Ryan J Separovich
• Exploiting pglB negative and positive Campylobacter jejuni to identify occupied and unoccupied sites of N-linked protein glycosylation - Joel A Cain

Each hub (Lorne, Sydney and Brisbane) will have a selection of posters available for viewing. 

Every poster will also be presented as a 3-minute video recording on the virtual conference platform.

• Understanding how azithromycin reduces asthma exacerbations and the underlying mechanisms of macrolides - Lisa M Jurak
• SWATH Quantification of blister fluid proteome from paediatric burn injuries using an integrated ion library - Tuo Zang
• Investigation of recombinant human factor IX posttranslational modification differences in fed-batch and perfusion processes of Chinese Hamster Ovary cells against plasma-derived factor IX - Dinora D Roche Recinos
• Proteogenomic analysis to identify cancer neo-antigens - Sonali V Mohan
• Glycoproteomics to determine site-specific autopolysialylation in human ST8 sialyltransferases - Ruby Pelingon
• Discovery of organelle membrane remodelling associated with CAV1/CAVIN1 in prostate cancer using integrative Protein and Lipid Organelle Profiling (iPLOP) - Harley Robinson
• The Role of RBBP7 in Maintaining Genomic Stability - Alejandra Fernandez
• The use of tetracycline-off system to study yeast with deficiency in both Ost3p and Ost6p - Chun Zhou
• A new, algorithm-based analysis workflow to explore non-traditional cereals for the presence of gluten-like proteins - Sophia Escobar-Correas
• Integrating SWATH-MS and Multi-omics Data Analysis to Study Impact of Gene Methylation on Protein Abundance - Manika Singh
• A sweet way of understanding and predicting viral zoonoses by comprehensive study of vertebrate host glycome using state-of-the-art glycomics - Abarna Vidya Mohana Murugan
• Identification and characterisation of sPEPs in Cryptococcus neoformans - Paige Erpf
• Rapidly Advance Quantitative Proteomics with a High-Throughput SWATH® Acquisition Solution - Christie Hunter
• Cryptosporidiosis modulates gut microbiome metabolism and the immune response in an infected host.   - Avinash Karpe
• Immunopeptidogenomics: harnessing RNA-seq to illuminate the dark immunopeptidome - Katherine E Scull
• The role of non-proteasomal proteases in antigen presentation in melanoma - Ritchlynn Mr Aranha
• Benchmarking targeted analysis pipelines in data-independent acquisition mass spectrometry-based immunopeptidomics - Mohammad Shahbazy
• Explorations of major eukaryotic “cell fate regulator” at the base of eukaryogenesis: the Pumilio homology superfamily in ‘basal’ eukaryote Giardia duodenalis - Balu Balan
• Proteomic profiling of human trophectoderm small extracellular vesicles reveal insights into implantation and remodeling through endometrial cell reprogramming - Qi Hui Poh
• Straight from the heart: Characterisation of cardiac extracellular vesicles - Bethany Claridge
• Multi-Omic exploration of human skeletal muscle after endurance training identifies extensive mitochondrial biogenesis and remodelling. - Nikeisha J Caruana
• Proteomics at scale: Experimental design and automated data management for large clinical cohorts - Ahmed Mohamed
• Evaluation of prmPASEF for multiplexed targeted proteomics - Pierre-Olivier Schmit
• High throughput proteomics - Application of dia-PASEF for short gradients - Adam Rainczuk
• Evaluation of prm-PASEF for multiplexed targeted proteomics. - Adam Rainczuk
• OneOmics™ Suite – Proteomics Processing Pipeline in the Cloud - Jeremy Potriquet
• Hexosaminidase disruption impedes the biosynthesis of paucimannosidic proteins in HL-60 cells - Julian Ugonotti
• Assessing an E. coli dataset by Top-down and Bottom-up proteomics using two-dimensional separation - Matthew J Fitzhenry
• Unexpected glycan signatures of the RCL of human corticosteroid-binding globulin impact its susceptibility to proteolytic cleavage - Anastasia Chernykh
• Sexual dimorphism in the liver’s response to intermittent fasting - Mark Larance
• Using smartphones to explore protein structures in XR - Neblina Sikta
• Proteomic characterisation of ancient Egyptian skin and bones - Dylan H Multari
• Abiotic stress factors in combination change the proteome of rice plants - Fatemeh Habibpourmehraban
• Proteome changes in the brain and eye of APP/PS1 mice illustrate molecular mechanisms of Alzheimer’s disease at the early stage - Liting Deng
• Temporal ordering of omics and multiomic events inferred from time-series data - Sandeep Ms Kaur
• Personalised phosphoproteomics of insulin action potentiated by exercise - Elise J Needham
• Quantitative mass spectrometry of cysteine redox post translational modifications in the diabetic heart - Alexander Rookyard
• Increasing the Ease of Use of Nanoflow with Plug and Play Low Flow Source - Keith Ashman
• Proteomic analysis of root tissue from two different varieties of rice with contrasting root architecture phenotypes - Paul A. Haynes
• Quantitative lipidomic analysis of soil-transmitted nematode Ascaris suum - Tao Wang
• Phosphoproteome profiling uncovers a key role for CDKs in TNF signaling - Maria C Tanzer
• Comparative study of excretory-secretory proteins released by Schistosoma mansoni-resistant, susceptible and naïve Biomphalaria glabrata - Conor Fogarty
• Strategies and Methodologies for Quantification of Senescence-Derived Biomarkers in Human Plasma - Birgit Schilling
• Band 3 Anion transport protein is up-regulated in high-grade serous ovarian cancer patients with acquired chemotherapy-resistant disease, as identified by MALDI-MSI - Tannith M Noye
• Effect of gas phase fractionation on protein identification rates in discovery proteomics of barley lines - Mahya Bahmani
• Proteogenomic study to investigate the impact of a transcription factor mutation on nutritional value and grain protein composition in barley - Angela Juhasz
• Discovery proteomic analysis of malt barley proteome – Understanding the impact of genetic variation on key malt quality traits. - Clare E O'Lone
• Quantifying gluten and allergens in a novel “low gluten” wheat variety - Mitchell G. Nye-Wood
• Confirmatory analysis of snake venoms by LC-HRMS for application to coronial toxicology - Joel P.A. Gummer
• C18ORF25 is a novel exercise-regulated AMPK substrate regulating metabolic adaptations - Yaan Kit Ng
• Development of quantitative assay for the detection of Proteinase A in beer using mass spectrometry - Brooke A Dilmetz
• Whole-cell proteomics of Streptomyces hygroscopicus NRRL 30439 reveals detailed temporal dynamics of secondary metabolism during submerged cell culture - Craig P Barry
• Australian BioCommons communities: engaging researchers at the national scale to understand bioinformatics challenges and deliver solutions - Johan Gustafsson
• The cancer stem cell marker DCLK1 influences extracellular vesicle biogenesis and cargo-selection in a kinase dependent manner promoting migration and adhesion processes in gastric cancer. - Annalisa Carli
• Tasmanian devil facial tumor-derived extracellular vesicles reveal mesenchymal transition markers and adhesion molecules related to metastasis - Richard Wilson
• Combining correlation network and enrichment analysis to help extract biological insights from complex proteomics experiments - Jemma X Wu
• Automated proteomics workflows for translational medicine: myocardial infarction and early origins of heart disease - Selvam Paramasivan
• Secreted midbody remnants are a class of extracellular vesicles molecularly distinct from exosomes and microparticles - Alin Rai
• Expanding proteomic coverage of barley via SWATH-MS with multiple proteolytic enzymes to assist malting accreditation of new varieties and brewing performance - Christopher H Caboche
• The multiple factors driving the protein composition of bee venom  - Ashley Nolan
• Label-Free Proteomics Performance with New Orbitrap Exploris 480 Mass Spectrometer with Single-Cell Sensitivity - Bruno Madio
• DIA performance in discovery and quantitation analysis on new Orbitrap Exploris mass spectrometers. - Enzo Huang

Lorne Evening Function - Lorne Central

Sydney Evening Function - Glasshouse Lawn

Brisbane Evening Function - Ballroom Foyer