LC-MS-based proteomics analysis has shown to be a powerful analytical tool for identification and quantification of thousands of proteins in complex biological samples. Moving forward from discovery to targeted quantitation in proteomics, there is a need for a robust mass spectrometry system and methods that provide reproducibility needed to analyze 1000s of samples without compromising on coverage and quantitation performance with ease of use for any level of analytical expertise. Here we present Orbitrap Exploris 480™ mass spectrometer coupled to the High-Field Asymmetric Waveform Ion Mobility Spectrometry System (FAIMS Pro™ Interface) for proteomics application on this new quadrupole orbitrap hybrid mass spectrometer. The performance of this small benchtop mass spectrometer is evaluated in a data-dependent acquisition (DDA) mode for sample injection amounts of just a single Hela cell up to 5000 HeLa cells (~1ug); providing sensitivity and performance required for analysis of large numbers of samples without compromising on sensitivity and proteome coverage. To demonstrate the sensitivity of the instrument we analyzed proteins from a single HeLa cell, as well as bulk digest equal to single cell levels. This instrument sensitivity enables identification of ~7000 protein groups from only a 200 ng of bulk HeLa digest and ~800 protein groups from a single Hela cell in 2hr gradient. The method performance was also evaluated across different instruments located in different laboratories.