Data independent acquisition ( mass spectrometry ( provides deep proteome analysis without the bias from peak intensity In addition to a more comprehensive coverage of identification, DIA also shows accurate label free quantification ( In these experiments we evaluated the DIA performance of both Thermo Scientific TM Orbitrap Exploris TM 480 mass spectrometer and Thermo Scientific TM Orbitrap Exploris TM 240 mass spectrometer for discovery and quantitative proteomics analysis.